Jurnal Sains dan Teknologi Laboratorium Medik

GAMBARAN KADAR CARBOHYDRATE ANTIGEN 19-9 ( CA 19-9 ) PADA PASIEN YANG DI CURIGAI KANKER PANKREAS DI RSUD ABDUL WAHAB SJAHRANIE SAMARINDA PADA TAHUN 2020-2022

2024-11-11T03:16:20+00:00

Pancreatic cancer is one of the most fatal cancers and the leading cause of cancer-related deaths. The survival rate for pancreatic cancer in the United States is reported to be 8%, which is another common type of cancer and in Indonesia itself pancreatic cancer is ranked 17th. Ca 19-9 is a cell produced by pancreatic exocrine gland cancer cells and can be detected in blood tests. The purpose of this study was to determine the Description of Ca 19-9 Levels in Pancreatic Cancer Patients at Abdul Wahab Sjahranie Hospital, Samarinda in 2020-2022. The research design used was the type of Analytical Observational method using Cross Sectional, meaning that the variables studied were only imposed once on a number of subjects who were the research samples. The results of the study were collected from medical records in the clinical pathology laboratory at Abdul Wahab Sjahranie Hospital, Samarinda in 2020-2022. From this study, it was found that there were 72 patients suspected of pancreatic cancer, where the majority were <55 years old, and 57% were male. Furthermore, based on the examination of Ca19-9 levels, it was found that 28 patients experienced levels above normal as much as 39%. The conclusion is that patients suspected of pancreatic cancer who underwent Ca 19-9 examination for the first time obtained results above normal levels, there were 28 patients with a percentage of 39% with an average age of > 65 years.

ANALISIS KADAR TIMBAL (Pb) PADA PEKERJA PENGELASAN DI DAERAH SAMARINDA SEBERANG DENGAN METODE SPEKTROFOTOMETER SERAPAN ATOM (SSA)

2024-11-11T03:16:20+00:00

Welding is a process of permanently joining metals by heating the metal to the melting point. This process will cause the accumulation of heavy metals in the air. UV and IR radiation is a kind of environmental exposure that can occur during welding. Long-term exposure to welding rays can cause problems with the lens of the eye including loss of transparency, discomfort in activities, itching in the eye area and breathing. Lead that is inhaled and enters the lungs will be associated with blood. Lead that is absorbed by the body will bind to red blood cells and then be distributed into the blood, extracellular fluid, and some soft places (liver, kidneys, and nerves) and mineral tissues (bones and teeth). The purpose of this study was to determine the blood lead levels of welding workers based on length of work, age, and working period with a normal limit value of <10 µg/dL according to Permenkes RI No. 70 of 2016. The type of research used is descriptive. Samples in the form of blood of shipyard welding workers in Samarinda Seberang area as many as 10 people The sampling technique in this study was quota sampling. Data were analyzed using univariate analysis. The results of this study showed that the average level of heavy metal lead in the blood of welding workers was 0.04 µg/dL with the limit of normal values in the blood <10 µg/dL. The conclusion in this study is that the blood lead levels of welding workers at the Shipyard in Samarinda Seberang City still meet the normal limit of <10 µg/dL according to Permenkes RI No. 70 of 2016.

Test the Effectiveness of Dayak Leeks (E. Palmifolia) Using Ethyl Acetate Solvent Against Staphylococcus Aureus Growth By the Well Method

2024-11-11T07:14:48+00:00

Dayak leek (Eleutherine palmifolia) is one of the plants that is often used by people for traditional
medicine in the Kalimantan region. Based on research, Dayak leeks contain alkaloids, flavonoids, saponins
and tannins which can function as antibiotics.
The aim of this research is to determine the effectiveness of Dayak leeks (Eleutherine palmifolia) using
ethyl acetate solvent on the growth of Staphylococcus aureus using the well method. The research design used
was true experimental, where this study used a treatment group and a positive control group (clindamycin 2
mcg).
The results of the research showed that in the test Dayak leek extract with concentrations of 60%,
65%, 70%, 75% and 80% formed an average diameter of the inhibition zone of 2 mm, 3.8 mm, 4 mm, 4.4 mm
and 7 mm in a row. And the control material clindamycin 2 mcg obtained an inhibition zone of 14 mm. So it
can be concluded that Dayak leeks can be used as an alternative to antibiotics, especially at high
concentrations.
Keywords: Dayak leek extract, Staphylococcus aureus, inhibitory power

Differences between Haemolysis and Non-Haemolysis Samples on Prothombin Time (PT) Values in East Kalimantan Health Polytechnic Students

2024-11-11T07:24:21+00:00

Haemolysis is the rupture of erythrocyte cells which results in the erythrocytes releasing haemoglobin into the serum or plasma. Haemolysed samples will affect pre-analytical errors in many laboratories. According to the Clinical Laboratory and Standards Institute (CLSI), in the Prothombin Time (PT) and Activated Partial Thromboplastin Time (APTT) testing guidelines, haemolysed samples should not be used for examination due to the potential activation of clotting factors and interference at the end of the measurement point. The purpose of this study was to determine the difference in the results of Prothombin Time (PT) examination in haemolysis samples with non-hemolysis. This type of research is a quasi-experimental design. The research design used is Nonequivalent Control Group Design, which is a design that takes measurements in treated and untreated groups. The samples used in this study were blood sodium citrate haemolysis and non-hemolysis as many as 39 specimens. Data normality analysis using Shapiro Wilk. Paired T-Test test to determine the difference between haemolysis and non-haemolysis samples. The results of this study showed that the average Prothombin Time value of non-hemolysis samples was 15 seconds, mild hemolysis samples were 9 seconds and moderate hemolysis was 8 seconds. In the univariate test results, the results were shortened in mild haemolysis and moderate haemolysis samples. In the bivariate test results obtained normal distributed results and obtained there is a difference in Prothombin Time values in hemolysis and non-hemolysis samples. The conclusion of this study is that there is a significant difference in hemolysis and non-hemolysis samples on the value of Prothombin Time with p value <0.05.

Keywords: Haemolysis, Photo Optic, Prothombin Time

Identifikasi Angka Kuman Udara Pada Ruang Pelayanan Di Puskesmas Wonorejo Samarinda

2024-11-11T07:27:01+00:00

Puskesmas merupakan pusat pelatihan tenaga kesehatan sebagai sarana pelayanan kesehatan, sehingga berpotensi menjadi tempat penularan penyakit dan memungkinkan pencemaran lingkungan. Kualitas udara yang tidak memenuhi persyaratan sebanding dengan angka kuman dalam ruang akan menyebabkan infeksi nosokomial. Tujuan penelitian untuk mengidentifikasi total angka kuman udara pada ruang pelayanan Puskesmas Wonorejo Samarinda.

Jenis penelitian adalah deskriptif dengan metode settle plate. Sampel yang digunakan adalah ruang pemeriksaan lansia, ruang pemeriksaan balita dan ruang tindakan pada tanggal 19 Maret – 23 Maret 2024. Hasil penelitian hari pertama, angka kuman pada ruang balita 713 CFU/m³, ruang lansia 918 CFU/m³, ruang tindakan 346 CFU/m³. Hari kedua, ruang balita 692 CFU/m³, ruang lansia 805 CFU/m³, ruang tindakan 437 CFU/m³. Hari ketiga, ruang balita 649 CFU/m³, ruang lansia 791 CFU/m³, ruang tindakan 430 CFU/m³. Hari keempat, ruang balita 289 CFU/m³, ruang lansia 480 CFU/m³, ruang tindakan 282 CFU/m³. Hari kelima, ruang balita 466 CFU/m³, ruang lansia 346 CFU/m³, ruang tindakan 261 CFU/m³. Kesimpulan penelitian adalah angka kuman udara pada ruang balita dan ruang lansia dari hari pertama hingga ketiga belum memenuhi syarat. Namun, hari keempat dan kelima memenuhi syarat. Sedangkan, angka kuman udara pada ruang tindakan dari hari pertama hingga kelima memenuhi syarat sesuai PERMENKES No.7 Tahun 2019,yaitu 200-500 CFU/m³.

Glucose, Ketone and Protein Profile in Diabetes Mellitus Patients Using Urine Strip Test at the Regional Health Laboratory UPTD of Samarinda City

2024-11-16T03:52:48+00:00

Diabetes mellitus is a disease of the metabolic system with the characteristics of hyperglycemia (an increase in blood sugar levels that exceed normal limits) which is caused by abnormalities in the body's insulin secretion, abnormalities in insulin action or even both. Currently, it is estimated that 25 million or around 9.3% of Indonesia's population in 2020 will experience impaired kidney function due to hypertension and diabetes. The aim of this study was to determine the profile of glucose, ketones and protein in diabetes mellitus sufferers using a urine strip test at the UPTD Regional Health Laboratory of Samarinda City. The type of research used is descriptive. The research population was prolanis patients who had been diagnosed with diabetes mellitus and had their urine checked at the UPTD Regional Health Laboratory of Samarinda City. The samples were Prolanis participants suffering from diabetes mellitus who were examined for urine glucose, ketones and protein using the urine strip test method. The results of the research were that in the urine glucose examination, 4 samples (13%) had 1 positive result and 5 samples (17%) had 2 positive results. In the urine ketone examination, 1 sample (3%) had a positive result of 1. In the urine protein examination, 3 samples (10%) had 1 positive result and 2 samples (7%) had 4 positive results.

A POTENSI ANTIKOAGULAN EKSTRAK MENGKUDU ( MORINDA CITRIFOLIA L. ) DENGAN METODE LEE WHITE DAN APUSAN DARAH

2024-11-19T04:55:08+00:00

Noni (Morinda citrifolia L.) is known to contain coumarin compounds which have the potential to act as anticoagulants to prevent blood clots. This study aims to test the anticoagulant potential of noni extract using the Lee White method and blood smears. This method was carried out on 15 blood samples to which noni extract was added at a concentration of 0.128 g and 0.192 g. Clotting time was measured using the Lee White method, while changes in blood morphology were observed through blood smears. The results showed that a concentration of 0.128 g of noni extract produced an average clotting time of 11 minutes 35 seconds, while at a concentration of 0.192 g the clotting time increased to an average of 14 minutes 37 seconds. Statistical tests showed that the increase in clotting time at both concentrations of noni extract was significant (p > 0.05), which supports the hypothesis that noni extract has an anticoagulant effect. In conclusion, noni extract has the potential as an anticoagulant agent that can prolong blood clotting time, so it has the potential to be used as an alternative natural anticoagulant for blood clotting therapy.

The Efektifitas Konsentrasi Pembuatan Handsanitizer Dari Ekstrak Daun Mengkudu (Morinda Citrifolia L.) Bentuk Gel Dalam Menghambat Pertumbuhan Staphylococcus Aureus

2024-11-28T08:15:01+00:00

Health is very important for life, one way to maintain a healthy body is by washing your hands using a hand sanitizer to kill bacteria. The aim of the research was to determine the effectiveness of hand sanitizer concentration from noni leaf extract (Morinda Citrifolia L.) in inhibiting the growth of Staphylococcus aureus. Staphylococcus aureus bacteria can cause skin and soft tissue infections such as pneumonia, osteomyelitis, and endocarditis. Natural hand sanitizer made from the Noni Plant (Morinda Citrifolia L.) contains alkaloids, flavonoids, saponins, tannins, which are antibacterial. The composition of the hand sanitizer is carbomer, TEA (triethanolamine), alcohol and glycerin. Research Method (Total Plate Count), count colonies on Mueller Hinton Agar media. The results of the research (Handsanitizer concentration 15%) grew colonies with an average number of (74 x 108 CFU/ml), (Handsanitizer concentration 20%) with an average number (73.54 x 108 CFU/ml). (Hand sanitizer concentration 25%) average amount (56.8 x 108 CFU/ml). (Handsanitizer concentration 30%) average amount (46.7 x 108 CFU/ml), (Handsanitizer concentration 35%) average amount (42.5 x 108 CFU/ml). Data analysis showed that based on the Anova test, the results showed that there was an influence of megkudu leaf extract hand sanitizer on the growth of Staphylococcus aureus. In the post hoc test, it was found that hand sanitizer with a concentration of 35% was the best concentration in inhibiting bacterial growth.