Jurnal Sains dan Teknologi Laboratorium Medik

Profil Hitung Jumlah dan Jenis Leukosit Pada Anak Terinfeksi Kecacingan Kota Samarinda

Ade Tasya Halim, Sresta Azahra — Thu, 02 Nov 2023 01:36:04 +0000

Helminthic infections are caused by helminth parasites. Helminthic worms are health problems that affect the defenses of the human immune system such as leukocytes. Children infected with helminthic worms are characterized by an increase in the number of eosinophils due to an immune response to helminthic infection. The purpose of knowing the profile of counting the number and type of leukocytes in children infected with worms.

Type of descriptive research with cross sectional design. The total population is 187 children with 9 children infected with worms in Samarinda City. This study was carried out by fecal examination (microscopic), leukocyte count examination (counting chamber) and leukocyte count (differential count). Analysis of data in univariate form.

The results of the study found 9 children (5%) infected with worms, 5 children (3%) male aged 6-12 years and 4 children (2%) female aged 8-10 years. Types of worm eggs obtained 5 children (56%) Ascaris lumbricoides, 2 children (22%) Trichuris trichiura and 2 children (22%) Hymenolepis nana. The number of leukocytes in infected children is within normal limits (4,500-13,500 μL). The type of abnormal leukocytes was obtained by 4 children (45%) who had elevated eosinophils. Conclusion The number of leukocytes in infected children is within normal limits and the type of leukocytes that have increased in the number of eosinophils (45%) indicates an immune response to helminthic infection.

Analysis of vitamin C levels in pineapple juice with the addition of natural sweeteners and artificial sweeteners based on the length of storage time

Linda Sari Dwi Setyaningsih, Eka Farpina, Ganea Qorry Aina — Thu, 02 Nov 2023 01:52:14 +0000

Vitamin C is one of the main vitamins needed by humans every day. Pineapple fruit contains vitamin C which can be obtained through direct consumption or consumed after processing, such as into juice. Vitamin C in fruit juice may decrease with the storage time, but the decrease in vitamin C levels can be suppressed by adding sweeteners. The purpose of this study was to determine the presence and absence of differences in vitamin C levels in pineapple juice with the addition of natural sweeteners and artificial sweeteners based on the length of storage time.

The type of research used is purely experimental. The study population was all pineapples harvested from pineapple plantations, Tani Bakti Village, Samboja District, Kutai Kartanegara Regency. The sample was pineapple juice made from Queen variety pineapple fruit with 4 variations of 10% concentration sweetener: sucrose, saccharin, cyclamate, and aspartame, and 1 variation of unsweetened control. With 4 variations of storage time namely 0, 30, 60, and 120 minutes. Analysis of vitamin C levels is carried out using the iodimetric titration method.

The results of the study found natural sweeteners and artificial sweeteners can suppress the decrease in vitamin C levels in pineapple juice when stored. The data from the study was then carried out static tests and obtained a value of p≤0.05 which showed that there was a difference in vitamin C levels in pineapple juice with the addition of natural sweeteners sucrose and artificial sweeteners saccharin, cyclamate, and aspartame during the storage period. After further static tests were carried out and it was found that based on all storage times there was a significant difference between sucrose-sweetened fruit juice, against control fruit juice with a p-value of ≤0.05. While from pineapple fruit juice other sweeteners did not show any significant difference from control pineapple fruit juice (unsweetened).

ANALYSIS OF UREUM LEVELS AND FASTING BLOOD GLUCOSE LEVELS IN CONVENTIONAL SMOKERS AND VAPE SMOKERS OF PRODUCTIVE AGE 18-21 YEARS OLD

Nazla Filda Apriliani — Thu, 02 Nov 2023 01:58:54 +0000

Conventional cigarettes and vape cigarettes contain nicotine. Nicotine in cigarettes has an influence on urea levels and blood glucose levels. The aim of this research is to determine urea levels and fasting blood glucose levels in conventional smokers and vape smokers of productive age 18-21 years. This type of research is descriptive and the variable in this research is 18-21 years old. The sampling technique uses purposive sampling technique. Samples were taken by taking venous blood which was then checked for urea levels and fasting blood glucose levels using a spectrophotometer. The data analysis used in this research is univariate analysis.The research results obtained: the average urea level of conventional smokers was 20.07 mg/dL and that of vape smokers was 20.85 mg/dL, the fasting blood glucose level of conventional smokers was 81.18 mg/dL and that of vape smokers was 77.04 mg/dL. The percentage of urea levels for conventional smokers is 93% normal, 7% is not normal, while vape smokers are 100% normal, not normal, 0%. The percentage of fasting blood glucose levels for conventional smokers is 60% normal, 40% abnormal, while for vape smokers it is 47% normal and 53% abnormal. Based on these results, both the average and percentage of urea levels and fasting blood glucose levels in conventional smokers and vape smokers of productive age 18-21 years are still within normal limits.

Keywords: Conventional cigarettes, Fasting blood glucose, Urea, Vape cigarettes

GAMBARAN KADAR SGOT DAN SGPT PADA PETANI YANG MENGGUNAKAN PESTISIDA DI DESA LOA JANAN ULU

Handa Nofianti Rosadi — Thu, 02 Nov 2023 02:13:41 +0000

ABSTRACT

Indonesia is an agricultural country where the agricultural sector is one of the main sources of the economy. However, farmers are worried about pest attacks, so they choose to use pesticides in order to increase agricultural yields. Therefore, in Indonesia, many farmers use pesticides, especially in East Kalimantan, precisely in the village of Loa Janan Ulu. The use of pesticides that violate the rules can endanger health, one of which is liver damage. Examination of SGOT (Serum Glutamic Oxoloacetic Transminase) and SGPT (Serum Glutamic Pyruvic Transminase) levels is one of the markers of liver damage, therefore this study aims to determine the average levels of SGOT and SGPT and the percentage of SGOT and SGPT levels that exceed the normal rate in farmers who use pesticides. The type of research used is descriptive with purposive sampling technique where the number of samples is 37 people. Based on the results, the highest average SGOT levels are at the age of 30-50 years (56 U/L) and work duration >10 years (55 U/L). While the highest average SGPT levels are aged 30-50 years (40 U/L) and have worked >10 years (39 U/L) and the percentage of SGOT levels is 95% and SGPT levels are 46% which exceeds the normal rate. It can be concluded that the average levels of SGOT and SGPT are highest at the age of 30-50 years and have worked for >10 years with a higher percentage of SGOT levels that exceed the normal rate than SGPT.

Keywords: Fermers, Pesticide, SGOT, SGPT

ANALYSIS OF CHOLINESTERASE ENZYME LEVELS IN FARMERS WHO USE PESTICIDES IN LOA JANAN ULU VILLAGE

Niken Syafitri — Thu, 02 Nov 2023 02:53:17 +0000

In Indonesia, many farmers use pesticides, especially in East Kalimantan, specifically in Loa Janan Ulu Village. Farmers use pesticides as an effort to reduce pest or insect attacks. The use of pesticides poses a risk to humans in the form of acute chronic poisoning and even death. Cholinesterase is an enzyme found in cell fluids whose job is to stop the action of acetylcholine by hydrolyzing it into choline and acetic acid. The aim of this research was to determine the levels of the cholinesterase enzyme in farmers who use pesticides in Loa Janan Ulu village. The type of research used was descriptive with a sample of 80 farmers in Loa Janan Ulu village who met the inclusion and exclusion criteria. The sampling technique was carried out using purposive sampling, with univariate analysis. The research results are based on the characteristics of respondents in the largest age group, namely 51-60 years (15 respondents), working period 21-30 years (14 respondents). The frequency of spraying was once a week (25 respondents). It can be concluded that in 33 samples the cholinesterase enzyme levels of 95% of farmers met normal standards and 5% did not meet normal standards.

OBSERVASI KADAR SGOT DAN SGPT PADA PEROKOK AKTIF BERDASARKAN USIA DEWASA DI WILAYAH BUKUAN RT 13

Annisa Putri Yasmeen — Thu, 02 Nov 2023 02:59:31 +0000

Smoking is a common bad habit worldwide and has the potential to cause a variety of serious diseases, including liver disorders. The enzymes SGOT and SGPT are used as sensitive indicators to measure liver function. This study aims to determine the levels of SGOT and SGPT in adult active smokers in the Bukuan RT 13 area.

This study used descriptive method and cross sectional research design. The study population consisted of 20 adult active smokers in Bukuan RT 13 with total sampling technique. Data obtained from the results of the examination of SGOT / SGPT levels and interviews then analyzed univariate.

The results showed that of the 20 respondents, all (100%) were active smokers with a smoking duration of more than 5 years. Abnormal SGOT levels were found in 12 people (60%), while SGPT was normal in 18 respondents (90%). The group who smoked 11-20 cigarettes per day had 5 respondents (25%) with normal SGOT results, while the group who smoked more than 20 cigarettes per day had 8 respondents (40%) with abnormal SGOT levels. Normal SGPT levels were found in 10 respondents (50%) from the group who smoked more than 20 cigarettes per day. The conclusion of this study is that some respondents showed abnormal SGOT and SGPT levels, which indicates the need for attention in the prevention and management of smoking-related health problems in active smokers with a smoking duration of more than 5 years.

GAMBARAN BERBAGAI MEREK HAND SANITIZER DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Staphylococcus Aureus SECARA IN-VITRO

sabri elbetan — Sun, 12 Nov 2023 04:08:53 +0000

Clean and Healthy Living Behaviour (PHBS) as an improvement in public health, one of which is washing hands or maintaining hand hygiene. Can be done in addition to using clean water can also use Hand sanitiser. In the community there are many brands of hand sanitiser, and this needs to be proven for its effectiveness. One of the things that can be done is to test its effectiveness on a laboratory scale. Bacteria that are commonly found as floranormal bacteria are Staphylococcus aureus. On a hospital scale this bacterium becomes a nosocomial problem as a methicillin-resistant bacterium or MRSA. The purpose of the study was to determine the effectiveness of various brands of hand sanitiser against the growth of Staphylococcus aureus ATCC 29213 bacteria. Test method using Kirby Baure with Mannitol Salt Agar media. Samples included 3 brands of Hand sanitiser which were carried out with 5 repetitions. The results of the study were that the 3 Hand sanitisers tested had various inhibition zones in inhibiting the growth of Staphylococcus aureus bacteria. The results of the Hand sanitizer inhibition test against the growth of Staphylococcus aureus, which is more effective is Hand sanitiser A than B and C, with a diameter of 19.66 mm, std. deviation 2.66 which indicates that type A Hand sanitiser is more effective and the distribution of its influence is more evenly distributed. Normality test results obtained Sig value. >0.05 the data is normally distributed, the results of the normality data test value Sig. >0.05 homogeneous data variance, One Way Anova test Sig. <0.05 there is a real difference, Post Hoc test results Hand sanitizer B and C Sig value. <0.05 there is a real difference.

The GAMBARAN EFEKTIVITAS ANTISEPTIK TERHADAP PERTUMBUHAN Candida albicans

Paul Hazler Ndara Milla — Sun, 12 Nov 2023 04:10:22 +0000

Antiseptics are antimicrobial substances that aim to inhibit or damage microorganisms on the surface of a living tissue. Candida albicans is the cause of candidiasis. This antiseptic has a role as an antimicrobial. The purpose of the study was to determine how minimal antiseptic concentrations inhibit the growth of Candida albicans and to determine how much antiseptic concentration kills the growth of Candida albicans. Type of experimental research using antiseptic samples. Solid dilution method Pour cup technique. The results of the study on antiseptic A concentrations of 20% (140 x 10² Cfu/ml), 40% (40.5 x 10² Cfu/ml), 60% (38 x 10² Cfu/ml), 80% (0 colonies), 100% (0 colonies). At antiseptic B concentration 20% (144.5 x 10² Cfu/ml), 40% (52 x 10² Cfu/ml), 60% (38 x 10² Cfu/ml), 80% (0 colonies), 100% (0 colonies). At antiseptic C concentration 20% (145.5 x 10² Cfu/ml), 40% (22 x 10² Cfu/ml), 60% (20 x 10² Cfu/ml), 80% (0 colonies), 100% (0 colonies). The conclusion of this study is a decrease in the number of Candida albicans colonies in antiseptic A concentration of 60% (38 x 10² Cfu/ml) antiseptic B concentration of 60% (38 x 10² Cfu/ml), antiseptic C concentration of 60% (20 x 10² Cfu/ml). Concentrations where there is no growth of Candida albicans colonies are found in antiseptic A, B and C concentrations of 80% (0 colonies).

Pemeriksaan Kadar HbCO Pada Relawan Pengatur Jalur Putar Balik Di Jalan Menggunakan Metode Spektrofotometer Uv-Vis

Rifky Ardani — Thu, 23 Nov 2023 08:53:06 +0000

The work of volunteers managing the U-Turn Route is very vulnerable to exposure to carbon monoxide, if it is inhaled by the body it will become CarboxyHemoglobin (HbCO). The aim of this research is to determine the levels of HbCO in volunteers' blood which regulates the U-turn pathway. The research population was 8 male people and the samples were veins. The results of this study showed that the lowest average HbCO level in the respondents' blood was 4.56% and the highest was 8.10%. Based on the age of respondents, 20-35 years old had an average level of 7.1%, aged 35-45 years had an average level of 5.8%. Based on work experience of more than 1 year, the average level is 6.2%, work experience of less than 1 year has an average level of 6.5%. Based on working time of 0-3 hours, it has an average level of 7.1%, working time of 4-7 hours has an average level of 5.6%. This research uses the Shapiro Wilk test and hypothesis testing using the correlation test with a significant value = α < 0.05. and obtained significant results, namely 100% abnormal.

ANALYSIS OF OXALIC ACID LEVELS IN SPINACH BASED ON SERVING TEMPERATURE

Mayang Fadma Sari — Thu, 21 Dec 2023 01:24:38 +0000

Spinach is one type of vegetable that contains a myriad of nutrients, but behind the many benefits contained in spinach there is also an inhibitory substance in the form of oxalic acid which is a chemical that can cause kidney stones, not only that excess oxalate in the body will also cause deficiency. or calcium deficiency. The purpose of this study was to determine the levels of oxalic acid in red and green spinach boiled water with different serving temperature variations. This type of research is descriptive, which is research conducted with the aim of describing or describing facts about the population systematically and accurately. With a total sample of 180 grams divided by 90 grams of red spinach and 90 grams of green spinach. The sampling technique used is Simple random sampling with a qualitative examination permanganometric titration method. The variable in this study was the oxalic acid content in red and green spinach based on serving temperature, with univariate data analysis. The results showed that the levels of oxalic acid in red spinach with temperature variations of 80^oC, 60^oC to 40^oC were 16.84mg/l, 19.28mg/l, 20.05mg/l. and green spinach with a temperature variation of 80^oC, 60^oC to 40^oC is 16.50mg/l, 17.68mg/l. 19.39mg/l. In this study, it can be concluded that the content of oxalic acid in boiled water of red spinach and green spinach based on the serving temperature of 80^oC, 60^oC to 40^oC has increased but is still at levels that are safe for consumption.

Analisis Hasil Pemeriksaan Kreatinin pada Sampel dengan Penambahan Hemolisat Berbagai Level

Salsa Amelia Putri — Wed, 10 Jan 2024 08:51:12 +0000

Examination of creatinine levels in the blood is one of the parameters used to assess kidney function because the concentration in plasma and its excretion in the urine in 24 hours is relatively constant. Hemolyzed plasma samples can affect creatinine levels with the examination results tending to decrease when compared to normal plasma examination results. Objective: To assess the percentage decrease or increase in mean, precision, accuracy, and clinical differences in creatinine assay by Jaffe Reaction Method using plasma samples with various levels of hemolysate added against samples without hemolysate added. Methods: Pure experiment (true experimental) with research samples in the form of venous blood of students and female students of the D-3 Medical Laboratory Technology Study Program of the East Kalimantan Ministry of Health Polytechnic as many as 40 samples. This research was conducted at the Hematology Laboratory and Clinical Chemistry Laboratory of the Poltekkes Kemenkes Kalimantan Timur. Data were obtained through measurement of the object of research after being treated in the form of the addition of various levels of hemolysate. Data analysis using Microsoft Excel application. Results: The mean decrease in creatinine test results of insignificant hemolysis and mild hemolysis samples against nonhemolyzed plasma samples was 6% and 8%. Accuracy value <5% in creatinine examination of insignificant hemolysis and mild hemolysis samples by -5% and -6%. The precision value >6% in the creatinine examination of insignificant hemolysis and mild hemolysis samples was 13.6% and 15.6%. Total error >15% TEa in the examination of insignificant hemolysis and mild hemolysis hemolysis samples was 27.2% and 31.2%. Conclusion: all the results of data analysis are outside the normal range and have a false decrease due to the addition of various levels of hemolysate, so the variation of insignificant hemolysis and mild hemolysis samples cannot be accepted or used in creatinine examination parameters.