Differences between Haemolysis and Non-Haemolysis Samples on Prothombin Time (PT) Values in East Kalimantan Health Polytechnic Students

Abstract

Haemolysis is the rupture of erythrocyte cells which results in the erythrocytes releasing haemoglobin into the serum or plasma. Haemolysed samples will affect pre-analytical errors in many laboratories.  According to the Clinical Laboratory and Standards Institute (CLSI), in the Prothombin Time (PT) and Activated Partial Thromboplastin Time (APTT) testing guidelines, haemolysed samples should not be used for examination due to the potential activation of clotting factors and interference at the end of the measurement point. The purpose of this study was to determine the difference in the results of Prothombin Time (PT) examination in haemolysis samples with non-hemolysis. This type of research is a quasi-experimental design. The research design used is Nonequivalent Control Group Design, which is a design that takes measurements in treated and untreated groups. The samples used in this study were blood sodium citrate haemolysis and non-hemolysis as many as 39 specimens. Data normality analysis using Shapiro Wilk. Paired T-Test test to determine the difference between haemolysis and non-haemolysis samples. The results of this study showed that the average Prothombin Time value of non-hemolysis samples was 15 seconds, mild hemolysis samples were 9 seconds and moderate hemolysis was 8 seconds. In the univariate test results, the results were shortened in mild haemolysis and moderate haemolysis samples. In the bivariate test results obtained normal distributed results and obtained there is a difference in Prothombin Time values in hemolysis and non-hemolysis samples. The conclusion of this study is that there is a significant difference in hemolysis and non-hemolysis samples on the value of Prothombin Time with p value <0.05.

Keywords: Haemolysis, Photo Optic, Prothombin Time